Towards a molecular taxonomic key of the Aurantioideae subfamily using chloroplastic SNP diagnostic markers of the main clades genotyped by competitive allele-specific PCR
Identifieur interne : 000022 ( Main/Exploration ); précédent : 000021; suivant : 000023Towards a molecular taxonomic key of the Aurantioideae subfamily using chloroplastic SNP diagnostic markers of the main clades genotyped by competitive allele-specific PCR
Auteurs : Amel Oueslati [Tunisie, France] ; Frederique Ollitrault [Espagne] ; Ghada Baraket [Tunisie] ; Amel Salhi-Hannachi [Tunisie] ; Luis Navarro [Espagne] ; Patrick Ollitrault [France]Source :
- BMC Genetics [ 1471-2156 ] ; 2016.
Abstract
Chloroplast DNA is a primary source of molecular variations for phylogenetic analysis of photosynthetic eukaryotes. However, the sequencing and analysis of multiple chloroplastic regions is difficult to apply to large collections or large samples of natural populations. The objective of our work was to demonstrate that a molecular taxonomic key based on easy, scalable and low-cost genotyping method should be developed from a set of Single Nucleotide Polymorphisms (SNPs) diagnostic of well-established clades. It was applied to the Aurantioideae subfamily, the largest group of the Rutaceae family that includes the cultivated citrus species.
The publicly available nucleotide sequences of eight plastid genomic regions were compared for 79 accessions of the Aurantioideae subfamily to search for SNPs revealing taxonomic differentiation at the inter-tribe, inter-subtribe, inter-genus and interspecific levels. Diagnostic SNPs (DSNPs) were found for 46 of the 54 clade levels analysed. Forty DSNPs were selected to develop KASPar markers and their taxonomic value was tested by genotyping 108 accessions of the Aurantioideae subfamily. Twenty-seven markers diagnostic of 24 clades were validated and they displayed a very high rate of transferability in the Aurantioideae subfamily (only 1.2 % of missing data on average). The UPGMA from the validated markers produced a cladistic organisation that was highly coherent with the previous phylogenetic analysis based on the sequence data of the eight plasmid regions. In particular, the monophyletic origin of the “true citrus” genera plus
We validated the concept that with well-established clades, DSNPs can be selected and efficiently transformed into competitive allele-specific PCR markers (KASPar method) allowing cost-effective highly efficient cladistic analysis in large collections at subfamily level. The robustness of this genotyping method is an additional decisive advantage for network collaborative research. The availability of WGS data for the main “true citrus” species should soon make it possible to develop a set of DSNP markers allowing very fine resolution of this very important horticultural group.
The online version of this article (doi:10.1186/s12863-016-0426-x) contains supplementary material, which is available to authorized users.
Url:
DOI: 10.1186/s12863-016-0426-x
PubMed: 27539067
PubMed Central: 4991024
Affiliations:
Links toward previous steps (curation, corpus...)
- to stream Pmc, to step Corpus: 000568
- to stream Pmc, to step Curation: 000567
- to stream Pmc, to step Checkpoint: 000017
- to stream Ncbi, to step Merge: 002149
- to stream Ncbi, to step Curation: 002149
- to stream Ncbi, to step Checkpoint: 002149
- to stream Main, to step Merge: 000022
- to stream Main, to step Curation: 000022
Le document en format XML
<record><TEI><teiHeader><fileDesc><titleStmt><title xml:lang="en">Towards a molecular taxonomic key of the Aurantioideae subfamily using chloroplastic SNP diagnostic markers of the main clades genotyped by competitive allele-specific PCR</title><author><name sortKey="Oueslati, Amel" sort="Oueslati, Amel" uniqKey="Oueslati A" first="Amel" last="Oueslati">Amel Oueslati</name><affiliation wicri:level="1"><nlm:aff id="Aff1">Laboratoire de Génétique Moléculaire, Immunologie et Biotechnologie LR99ES12, Faculté des Sciences de Tunis (FST), Université de Tunis El Manar, Campus Universitaire, El Manar-Tunis, 2092 Tunisia</nlm:aff><country xml:lang="fr">Tunisie</country><wicri:regionArea>Laboratoire de Génétique Moléculaire, Immunologie et Biotechnologie LR99ES12, Faculté des Sciences de Tunis (FST), Université de Tunis El Manar, Campus Universitaire, El Manar-Tunis</wicri:regionArea><wicri:noRegion>El Manar-Tunis</wicri:noRegion></affiliation><affiliation wicri:level="3"><nlm:aff id="Aff3">UMR Agap, CIRAD, Petit-Bourg, F-97170 Guadeloupe France</nlm:aff><country wicri:rule="zip">France</country><wicri:regionArea>UMR Agap, CIRAD, Petit-Bourg, F-97170 Guadeloupe France</wicri:regionArea><placeName><region type="region" nuts="2">Guadeloupe</region><settlement type="city">Guadeloupe France</settlement></placeName></affiliation></author><author><name sortKey="Ollitrault, Frederique" sort="Ollitrault, Frederique" uniqKey="Ollitrault F" first="Frederique" last="Ollitrault">Frederique Ollitrault</name><affiliation wicri:level="1"><nlm:aff id="Aff2">Centro de Protección Vegetal y Biotecnología, Instituto Valenciano de Investigaciones Agrarias (IVIA), Moncada, 46113 Valencia Spain</nlm:aff><country>Espagne</country><placeName><region type="province">Province de Valence</region></placeName><wicri:regionArea>Centro de Protección Vegetal y Biotecnología, Instituto Valenciano de Investigaciones Agrarias (IVIA), Moncada</wicri:regionArea><wicri:noRegion>Moncada</wicri:noRegion></affiliation></author><author><name sortKey="Baraket, Ghada" sort="Baraket, Ghada" uniqKey="Baraket G" first="Ghada" last="Baraket">Ghada Baraket</name><affiliation wicri:level="1"><nlm:aff id="Aff1">Laboratoire de Génétique Moléculaire, Immunologie et Biotechnologie LR99ES12, Faculté des Sciences de Tunis (FST), Université de Tunis El Manar, Campus Universitaire, El Manar-Tunis, 2092 Tunisia</nlm:aff><country xml:lang="fr">Tunisie</country><wicri:regionArea>Laboratoire de Génétique Moléculaire, Immunologie et Biotechnologie LR99ES12, Faculté des Sciences de Tunis (FST), Université de Tunis El Manar, Campus Universitaire, El Manar-Tunis</wicri:regionArea><wicri:noRegion>El Manar-Tunis</wicri:noRegion></affiliation></author><author><name sortKey="Salhi Hannachi, Amel" sort="Salhi Hannachi, Amel" uniqKey="Salhi Hannachi A" first="Amel" last="Salhi-Hannachi">Amel Salhi-Hannachi</name><affiliation wicri:level="1"><nlm:aff id="Aff1">Laboratoire de Génétique Moléculaire, Immunologie et Biotechnologie LR99ES12, Faculté des Sciences de Tunis (FST), Université de Tunis El Manar, Campus Universitaire, El Manar-Tunis, 2092 Tunisia</nlm:aff><country xml:lang="fr">Tunisie</country><wicri:regionArea>Laboratoire de Génétique Moléculaire, Immunologie et Biotechnologie LR99ES12, Faculté des Sciences de Tunis (FST), Université de Tunis El Manar, Campus Universitaire, El Manar-Tunis</wicri:regionArea><wicri:noRegion>El Manar-Tunis</wicri:noRegion></affiliation></author><author><name sortKey="Navarro, Luis" sort="Navarro, Luis" uniqKey="Navarro L" first="Luis" last="Navarro">Luis Navarro</name><affiliation wicri:level="1"><nlm:aff id="Aff2">Centro de Protección Vegetal y Biotecnología, Instituto Valenciano de Investigaciones Agrarias (IVIA), Moncada, 46113 Valencia Spain</nlm:aff><country>Espagne</country><placeName><region type="province">Province de Valence</region></placeName><wicri:regionArea>Centro de Protección Vegetal y Biotecnología, Instituto Valenciano de Investigaciones Agrarias (IVIA), Moncada</wicri:regionArea><wicri:noRegion>Moncada</wicri:noRegion></affiliation></author><author><name sortKey="Ollitrault, Patrick" sort="Ollitrault, Patrick" uniqKey="Ollitrault P" first="Patrick" last="Ollitrault">Patrick Ollitrault</name><affiliation wicri:level="3"><nlm:aff id="Aff3">UMR Agap, CIRAD, Petit-Bourg, F-97170 Guadeloupe France</nlm:aff><country wicri:rule="zip">France</country><wicri:regionArea>UMR Agap, CIRAD, Petit-Bourg, F-97170 Guadeloupe France</wicri:regionArea><placeName><region type="region" nuts="2">Guadeloupe</region><settlement type="city">Guadeloupe France</settlement></placeName></affiliation></author></titleStmt><publicationStmt><idno type="wicri:source">PMC</idno><idno type="pmid">27539067</idno><idno type="pmc">4991024</idno><idno type="url">http://www.ncbi.nlm.nih.gov/pmc/articles/PMC4991024</idno><idno type="RBID">PMC:4991024</idno><idno type="doi">10.1186/s12863-016-0426-x</idno><date when="2016">2016</date><idno type="wicri:Area/Pmc/Corpus">000568</idno><idno type="wicri:Area/Pmc/Curation">000567</idno><idno type="wicri:Area/Pmc/Checkpoint">000017</idno><idno type="wicri:Area/Ncbi/Merge">002149</idno><idno type="wicri:Area/Ncbi/Curation">002149</idno><idno type="wicri:Area/Ncbi/Checkpoint">002149</idno><idno type="wicri:Area/Main/Merge">000022</idno><idno type="wicri:Area/Main/Curation">000022</idno><idno type="wicri:Area/Main/Exploration">000022</idno></publicationStmt><sourceDesc><biblStruct><analytic><title xml:lang="en" level="a" type="main">Towards a molecular taxonomic key of the Aurantioideae subfamily using chloroplastic SNP diagnostic markers of the main clades genotyped by competitive allele-specific PCR</title><author><name sortKey="Oueslati, Amel" sort="Oueslati, Amel" uniqKey="Oueslati A" first="Amel" last="Oueslati">Amel Oueslati</name><affiliation wicri:level="1"><nlm:aff id="Aff1">Laboratoire de Génétique Moléculaire, Immunologie et Biotechnologie LR99ES12, Faculté des Sciences de Tunis (FST), Université de Tunis El Manar, Campus Universitaire, El Manar-Tunis, 2092 Tunisia</nlm:aff><country xml:lang="fr">Tunisie</country><wicri:regionArea>Laboratoire de Génétique Moléculaire, Immunologie et Biotechnologie LR99ES12, Faculté des Sciences de Tunis (FST), Université de Tunis El Manar, Campus Universitaire, El Manar-Tunis</wicri:regionArea><wicri:noRegion>El Manar-Tunis</wicri:noRegion></affiliation><affiliation wicri:level="3"><nlm:aff id="Aff3">UMR Agap, CIRAD, Petit-Bourg, F-97170 Guadeloupe France</nlm:aff><country wicri:rule="zip">France</country><wicri:regionArea>UMR Agap, CIRAD, Petit-Bourg, F-97170 Guadeloupe France</wicri:regionArea><placeName><region type="region" nuts="2">Guadeloupe</region><settlement type="city">Guadeloupe France</settlement></placeName></affiliation></author><author><name sortKey="Ollitrault, Frederique" sort="Ollitrault, Frederique" uniqKey="Ollitrault F" first="Frederique" last="Ollitrault">Frederique Ollitrault</name><affiliation wicri:level="1"><nlm:aff id="Aff2">Centro de Protección Vegetal y Biotecnología, Instituto Valenciano de Investigaciones Agrarias (IVIA), Moncada, 46113 Valencia Spain</nlm:aff><country>Espagne</country><placeName><region type="province">Province de Valence</region></placeName><wicri:regionArea>Centro de Protección Vegetal y Biotecnología, Instituto Valenciano de Investigaciones Agrarias (IVIA), Moncada</wicri:regionArea><wicri:noRegion>Moncada</wicri:noRegion></affiliation></author><author><name sortKey="Baraket, Ghada" sort="Baraket, Ghada" uniqKey="Baraket G" first="Ghada" last="Baraket">Ghada Baraket</name><affiliation wicri:level="1"><nlm:aff id="Aff1">Laboratoire de Génétique Moléculaire, Immunologie et Biotechnologie LR99ES12, Faculté des Sciences de Tunis (FST), Université de Tunis El Manar, Campus Universitaire, El Manar-Tunis, 2092 Tunisia</nlm:aff><country xml:lang="fr">Tunisie</country><wicri:regionArea>Laboratoire de Génétique Moléculaire, Immunologie et Biotechnologie LR99ES12, Faculté des Sciences de Tunis (FST), Université de Tunis El Manar, Campus Universitaire, El Manar-Tunis</wicri:regionArea><wicri:noRegion>El Manar-Tunis</wicri:noRegion></affiliation></author><author><name sortKey="Salhi Hannachi, Amel" sort="Salhi Hannachi, Amel" uniqKey="Salhi Hannachi A" first="Amel" last="Salhi-Hannachi">Amel Salhi-Hannachi</name><affiliation wicri:level="1"><nlm:aff id="Aff1">Laboratoire de Génétique Moléculaire, Immunologie et Biotechnologie LR99ES12, Faculté des Sciences de Tunis (FST), Université de Tunis El Manar, Campus Universitaire, El Manar-Tunis, 2092 Tunisia</nlm:aff><country xml:lang="fr">Tunisie</country><wicri:regionArea>Laboratoire de Génétique Moléculaire, Immunologie et Biotechnologie LR99ES12, Faculté des Sciences de Tunis (FST), Université de Tunis El Manar, Campus Universitaire, El Manar-Tunis</wicri:regionArea><wicri:noRegion>El Manar-Tunis</wicri:noRegion></affiliation></author><author><name sortKey="Navarro, Luis" sort="Navarro, Luis" uniqKey="Navarro L" first="Luis" last="Navarro">Luis Navarro</name><affiliation wicri:level="1"><nlm:aff id="Aff2">Centro de Protección Vegetal y Biotecnología, Instituto Valenciano de Investigaciones Agrarias (IVIA), Moncada, 46113 Valencia Spain</nlm:aff><country>Espagne</country><placeName><region type="province">Province de Valence</region></placeName><wicri:regionArea>Centro de Protección Vegetal y Biotecnología, Instituto Valenciano de Investigaciones Agrarias (IVIA), Moncada</wicri:regionArea><wicri:noRegion>Moncada</wicri:noRegion></affiliation></author><author><name sortKey="Ollitrault, Patrick" sort="Ollitrault, Patrick" uniqKey="Ollitrault P" first="Patrick" last="Ollitrault">Patrick Ollitrault</name><affiliation wicri:level="3"><nlm:aff id="Aff3">UMR Agap, CIRAD, Petit-Bourg, F-97170 Guadeloupe France</nlm:aff><country wicri:rule="zip">France</country><wicri:regionArea>UMR Agap, CIRAD, Petit-Bourg, F-97170 Guadeloupe France</wicri:regionArea><placeName><region type="region" nuts="2">Guadeloupe</region><settlement type="city">Guadeloupe France</settlement></placeName></affiliation></author></analytic><series><title level="j">BMC Genetics</title><idno type="eISSN">1471-2156</idno><imprint><date when="2016">2016</date></imprint></series></biblStruct></sourceDesc></fileDesc><profileDesc><textClass></textClass></profileDesc></teiHeader><front><div type="abstract" xml:lang="en"><sec><title>Background</title><p>Chloroplast DNA is a primary source of molecular variations for phylogenetic analysis of photosynthetic eukaryotes. However, the sequencing and analysis of multiple chloroplastic regions is difficult to apply to large collections or large samples of natural populations. The objective of our work was to demonstrate that a molecular taxonomic key based on easy, scalable and low-cost genotyping method should be developed from a set of Single Nucleotide Polymorphisms (SNPs) diagnostic of well-established clades. It was applied to the Aurantioideae subfamily, the largest group of the Rutaceae family that includes the cultivated citrus species.</p></sec><sec><title>Results</title><p>The publicly available nucleotide sequences of eight plastid genomic regions were compared for 79 accessions of the Aurantioideae subfamily to search for SNPs revealing taxonomic differentiation at the inter-tribe, inter-subtribe, inter-genus and interspecific levels. Diagnostic SNPs (DSNPs) were found for 46 of the 54 clade levels analysed. Forty DSNPs were selected to develop KASPar markers and their taxonomic value was tested by genotyping 108 accessions of the Aurantioideae subfamily. Twenty-seven markers diagnostic of 24 clades were validated and they displayed a very high rate of transferability in the Aurantioideae subfamily (only 1.2 % of missing data on average). The UPGMA from the validated markers produced a cladistic organisation that was highly coherent with the previous phylogenetic analysis based on the sequence data of the eight plasmid regions. In particular, the monophyletic origin of the “true citrus” genera plus <italic>Oxanthera</italic> was validated. However, some clarification remains necessary regarding the organisation of the other wild species of the Citreae tribe.</p></sec><sec><title>Conclusions</title><p>We validated the concept that with well-established clades, DSNPs can be selected and efficiently transformed into competitive allele-specific PCR markers (KASPar method) allowing cost-effective highly efficient cladistic analysis in large collections at subfamily level. The robustness of this genotyping method is an additional decisive advantage for network collaborative research. The availability of WGS data for the main “true citrus” species should soon make it possible to develop a set of DSNP markers allowing very fine resolution of this very important horticultural group.</p></sec><sec><title>Electronic supplementary material</title><p>The online version of this article (doi:10.1186/s12863-016-0426-x) contains supplementary material, which is available to authorized users.</p></sec></div></front><back><div1 type="bibliography"><listBibl><biblStruct><analytic><author><name sortKey="Raubeson, La" uniqKey="Raubeson L">LA Raubeson</name></author><author><name sortKey="Jansen, Rk" uniqKey="Jansen R">RK Jansen</name></author></analytic></biblStruct><biblStruct><analytic><author><name sortKey="Wolfe, Kh" uniqKey="Wolfe K">KH Wolfe</name></author><author><name sortKey="Li, Wh" uniqKey="Li W">WH Li</name></author><author><name sortKey="Sharp, Pm" uniqKey="Sharp P">PM Sharp</name></author></analytic></biblStruct><biblStruct><analytic><author><name sortKey="Gao, X" uniqKey="Gao X">X Gao</name></author><author><name sortKey="Zhu, Yp" uniqKey="Zhu Y">YP Zhu</name></author><author><name sortKey="Wu, Bc" uniqKey="Wu B">BC Wu</name></author><author><name sortKey="Zhao, Ym" uniqKey="Zhao Y">YM Zhao</name></author><author><name sortKey="Chen, Jq" uniqKey="Chen J">JQ Chen</name></author><author><name sortKey="Hang, Yy" uniqKey="Hang Y">YY Hang</name></author></analytic></biblStruct><biblStruct><analytic><author><name sortKey="Li, Jh" uniqKey="Li J">JH Li</name></author></analytic></biblStruct><biblStruct><analytic><author><name sortKey="Wilson, Ca" uniqKey="Wilson C">CA Wilson</name></author></analytic></biblStruct><biblStruct><analytic><author><name sortKey="Peterson, Pm" uniqKey="Peterson P">PM Peterson</name></author><author><name sortKey="Romaschenko, K" uniqKey="Romaschenko K">K Romaschenko</name></author><author><name sortKey="Johnson, G" uniqKey="Johnson G">G Johnson</name></author></analytic></biblStruct><biblStruct><analytic><author><name sortKey="Hilu, Kw" uniqKey="Hilu K">KW Hilu</name></author><author><name sortKey="Black, C" uniqKey="Black C">C Black</name></author><author><name sortKey="Diouf, D" uniqKey="Diouf D">D Diouf</name></author><author><name sortKey="Burleigh, Jg" uniqKey="Burleigh J">JG Burleigh</name></author></analytic></biblStruct><biblStruct><analytic><author><name sortKey="Kim, Kj" uniqKey="Kim K">KJ Kim</name></author><author><name sortKey="Jansen, Rk" uniqKey="Jansen R">RK Jansen</name></author></analytic></biblStruct><biblStruct><analytic><author><name sortKey="Dong, W" uniqKey="Dong W">W Dong</name></author><author><name sortKey="Liu, J" uniqKey="Liu J">J Liu</name></author><author><name sortKey="Yu, J" uniqKey="Yu J">J Yu</name></author><author><name sortKey="Wang, L" uniqKey="Wang L">L Wang</name></author><author><name sortKey="Zhou, S" uniqKey="Zhou S">S Zhou</name></author></analytic></biblStruct><biblStruct><analytic><author><name sortKey="Carbonell Caballero, J" uniqKey="Carbonell Caballero J">J Carbonell-Caballero</name></author><author><name sortKey="Alonso, R" uniqKey="Alonso R">R Alonso</name></author><author><name sortKey="Ibanez, V" uniqKey="Ibanez V">V Ibanez</name></author><author><name sortKey="Terol, J" uniqKey="Terol J">J Terol</name></author><author><name sortKey="Talon, M" uniqKey="Talon M">M Talon</name></author><author><name sortKey="Dopazo, J" uniqKey="Dopazo J">J Dopazo</name></author></analytic></biblStruct><biblStruct><analytic><author><name sortKey="Engler, A" uniqKey="Engler A">A Engler</name></author></analytic></biblStruct><biblStruct><analytic><author><name sortKey="Thorne, Rf" uniqKey="Thorne R">RF Thorne</name></author></analytic></biblStruct><biblStruct><analytic><author><name sortKey="Swingle, Wt" uniqKey="Swingle W">WT Swingle</name></author><author><name sortKey="Reece, Pc" uniqKey="Reece P">PC Reece</name></author></analytic></biblStruct><biblStruct><analytic><author><name sortKey="Krueger, Rr" uniqKey="Krueger R">RR Krueger</name></author><author><name sortKey="Navarro, L" uniqKey="Navarro L">L Navarro</name></author></analytic></biblStruct><biblStruct><analytic><author><name sortKey="Federici, Ct" uniqKey="Federici C">CT Federici</name></author><author><name sortKey="Fang, Dq" uniqKey="Fang D">DQ Fang</name></author><author><name sortKey="Scora, Rw" uniqKey="Scora R">RW Scora</name></author><author><name sortKey="Roose, Ml" uniqKey="Roose M">ML Roose</name></author></analytic></biblStruct><biblStruct><analytic><author><name sortKey="Nicolosi, E" uniqKey="Nicolosi E">E Nicolosi</name></author><author><name sortKey="Deng, Zn" uniqKey="Deng Z">ZN Deng</name></author><author><name sortKey="Gentile, A" uniqKey="Gentile A">A Gentile</name></author><author><name sortKey="La Malfa, S" uniqKey="La Malfa S">S La Malfa</name></author><author><name sortKey="Continella, G" uniqKey="Continella G">G Continella</name></author><author><name sortKey="Tribulato, E" uniqKey="Tribulato E">E Tribulato</name></author></analytic></biblStruct><biblStruct><analytic><author><name sortKey="Barkley, Na" uniqKey="Barkley N">NA Barkley</name></author><author><name sortKey="Roose, Ml" uniqKey="Roose M">ML Roose</name></author><author><name sortKey="Krueger, Rr" uniqKey="Krueger R">RR Krueger</name></author><author><name sortKey="Federici, Ct" uniqKey="Federici C">CT Federici</name></author></analytic></biblStruct><biblStruct><analytic><author><name sortKey="Froelicher, Y" uniqKey="Froelicher Y">Y Froelicher</name></author><author><name sortKey="Mouhaya, W" uniqKey="Mouhaya W">W Mouhaya</name></author><author><name sortKey="Bassene, Jb" uniqKey="Bassene J">JB Bassene</name></author><author><name sortKey="Costantino, G" uniqKey="Costantino G">G Costantino</name></author><author><name sortKey="Kamiri, M" uniqKey="Kamiri M">M Kamiri</name></author></analytic></biblStruct><biblStruct><analytic><author><name sortKey="Garcia Lor, A" uniqKey="Garcia Lor A">A Garcia-Lor</name></author><author><name sortKey="Luro, F" uniqKey="Luro F">F Luro</name></author><author><name sortKey="Navarro, L" uniqKey="Navarro L">L Navarro</name></author><author><name sortKey="Ollitrault, P" uniqKey="Ollitrault P">P Ollitrault</name></author></analytic></biblStruct><biblStruct><analytic><author><name sortKey="Garcia Lor, A" uniqKey="Garcia Lor A">A Garcia-Lor</name></author><author><name sortKey="Curk, F" uniqKey="Curk F">F Curk</name></author><author><name sortKey="Snoussi Trifa, H" uniqKey="Snoussi Trifa H">H Snoussi-Trifa</name></author><author><name sortKey="Morillon, R" uniqKey="Morillon R">R Morillon</name></author><author><name sortKey="Ancillo, G" uniqKey="Ancillo G">G Ancillo</name></author><author><name sortKey="Luro, F" uniqKey="Luro F">F Luro</name></author></analytic></biblStruct><biblStruct><analytic><author><name sortKey="Ollitrault, P" uniqKey="Ollitrault P">P Ollitrault</name></author><author><name sortKey="Terol, J" uniqKey="Terol J">J Terol</name></author><author><name sortKey="Garcia Lor, A" uniqKey="Garcia Lor A">A Garcia-Lor</name></author><author><name sortKey="Aurelie, B" uniqKey="Aurelie B">B Aurélie</name></author><author><name sortKey="Aurelie, C" uniqKey="Aurelie C">C Aurélie</name></author><author><name sortKey="Yann, F" uniqKey="Yann F">F Yann</name></author></analytic></biblStruct><biblStruct><analytic><author><name sortKey="Curk, F" uniqKey="Curk F">F Curk</name></author><author><name sortKey="Ancillo, G" uniqKey="Ancillo G">G Ancillo</name></author><author><name sortKey="Garcia Lor, A" uniqKey="Garcia Lor A">A Garcia-Lor</name></author><author><name sortKey="Luro, F" uniqKey="Luro F">F Luro</name></author><author><name sortKey="Perrier, X" uniqKey="Perrier X">X Perrier</name></author><author><name sortKey="Jacquemoud Collet, Jp" uniqKey="Jacquemoud Collet J">JP Jacquemoud-Collet</name></author></analytic></biblStruct><biblStruct><analytic><author><name sortKey="Curk, F" uniqKey="Curk F">F Curk</name></author><author><name sortKey="Ancillo, G" uniqKey="Ancillo G">G Ancillo</name></author><author><name sortKey="Ollitrault, F" uniqKey="Ollitrault F">F Ollitrault</name></author><author><name sortKey="Perrier, X" uniqKey="Perrier X">X Perrier</name></author><author><name sortKey="Jacquemoud Collet, Jp" uniqKey="Jacquemoud Collet J">JP Jacquemoud-Collet</name></author><author><name sortKey="Garcia Lor, A" uniqKey="Garcia Lor A">A Garcia-Lor</name></author></analytic></biblStruct><biblStruct><analytic><author><name sortKey="Tanaka, T" uniqKey="Tanaka T">T Tanaka</name></author></analytic></biblStruct><biblStruct><analytic><author><name sortKey="Mabberley, Dj" uniqKey="Mabberley D">DJ Mabberley</name></author></analytic></biblStruct><biblStruct><analytic><author><name sortKey="Olmstead, Rg" uniqKey="Olmstead R">RG Olmstead</name></author><author><name sortKey="Palmer, Jd" uniqKey="Palmer J">JD Palmer</name></author></analytic></biblStruct><biblStruct><analytic><author><name sortKey="Samuel, R" uniqKey="Samuel R">R Samuel</name></author><author><name sortKey="Ehrenddorfer, F" uniqKey="Ehrenddorfer F">F Ehrenddorfer</name></author><author><name sortKey="Chase, Mw" uniqKey="Chase M">MW Chase</name></author><author><name sortKey="Greger, H" uniqKey="Greger H">H Greger</name></author></analytic></biblStruct><biblStruct><analytic><author><name sortKey="Araujo, Ef" uniqKey="Araujo E">EF Araujo</name></author><author><name sortKey="Queiroz, Lp" uniqKey="Queiroz L">LP Queiroz</name></author><author><name sortKey="Machado, Ma" uniqKey="Machado M">MA Machado</name></author></analytic></biblStruct><biblStruct><analytic><author><name sortKey="Morton, C" uniqKey="Morton C">C Morton</name></author><author><name sortKey="Grant, M" uniqKey="Grant M">M Grant</name></author><author><name sortKey="Blackmore, S" uniqKey="Blackmore S">S Blackmore</name></author></analytic></biblStruct><biblStruct><analytic><author><name sortKey="Morton, C" uniqKey="Morton C">C Morton</name></author></analytic></biblStruct><biblStruct><analytic><author><name sortKey="Bayer, R" uniqKey="Bayer R">R Bayer</name></author><author><name sortKey="Mabberley, D" uniqKey="Mabberley D">D Mabberley</name></author><author><name sortKey="Morton, Cm" uniqKey="Morton C">CM Morton</name></author><author><name sortKey="Cathy, H" uniqKey="Cathy H">H Cathy</name></author><author><name sortKey="Sharma, I" uniqKey="Sharma I">I Sharma</name></author><author><name sortKey="Pfeil, Be" uniqKey="Pfeil B">BE Pfeil</name></author></analytic></biblStruct><biblStruct><analytic><author><name sortKey="Lu, Z" uniqKey="Lu Z">Z Lu</name></author><author><name sortKey="Zhou, Z" uniqKey="Zhou Z">Z Zhou</name></author><author><name sortKey="Xie, R" uniqKey="Xie R">R Xie</name></author></analytic></biblStruct><biblStruct><analytic><author><name sortKey="Bausher, M" uniqKey="Bausher M">M Bausher</name></author><author><name sortKey="Singh, N" uniqKey="Singh N">N Singh</name></author><author><name sortKey="Lee, S" uniqKey="Lee S">S Lee</name></author><author><name sortKey="Jansen, R" uniqKey="Jansen R">R Jansen</name></author><author><name sortKey="Daniell, H" uniqKey="Daniell H">H Daniell</name></author></analytic></biblStruct><biblStruct><analytic><author><name sortKey="Green, Rm" uniqKey="Green R">RM Green</name></author><author><name sortKey="Vardi, A" uniqKey="Vardi A">A Vardi</name></author><author><name sortKey="Galun, E" uniqKey="Galun E">E Galun</name></author></analytic></biblStruct><biblStruct><analytic><author><name sortKey="Yamamoto, M" uniqKey="Yamamoto M">M Yamamoto</name></author><author><name sortKey="Kobayashi, S" uniqKey="Kobayashi S">S Kobayashi</name></author><author><name sortKey="Nakamura, Y" uniqKey="Nakamura Y">Y Nakamura</name></author><author><name sortKey="Yamada, Y" uniqKey="Yamada Y">Y Yamada</name></author></analytic></biblStruct><biblStruct><analytic><author><name sortKey="Penjor, T" uniqKey="Penjor T">T Penjor</name></author><author><name sortKey="Yamamoto, M" uniqKey="Yamamoto M">M Yamamoto</name></author><author><name sortKey="Uehara, M" uniqKey="Uehara M">M Uehara</name></author><author><name sortKey="Ide, M" uniqKey="Ide M">M Ide</name></author><author><name sortKey="Matsumoto, N" uniqKey="Matsumoto N">N Matsumoto</name></author><author><name sortKey="Matsumoto, R" uniqKey="Matsumoto R">R Matsumoto</name></author><author><name sortKey="Nagano, Y" uniqKey="Nagano Y">Y Nagano</name></author></analytic></biblStruct><biblStruct><analytic><author><name sortKey="Li, Y" uniqKey="Li Y">Y Li</name></author><author><name sortKey="Cheng, Y" uniqKey="Cheng Y">Y Cheng</name></author><author><name sortKey="Tao, N" uniqKey="Tao N">N Tao</name></author><author><name sortKey="Deng, X" uniqKey="Deng X">X Deng</name></author></analytic></biblStruct><biblStruct><analytic><author><name sortKey="Jena, Sn" uniqKey="Jena S">SN Jena</name></author><author><name sortKey="Kumar, S" uniqKey="Kumar S">S Kumar</name></author><author><name sortKey="Nair, Kn" uniqKey="Nair K">KN Nair</name></author></analytic></biblStruct><biblStruct><analytic><author><name sortKey="Yamamoto, M" uniqKey="Yamamoto M">M Yamamoto</name></author><author><name sortKey="Tsuchimochi, Y" uniqKey="Tsuchimochi Y">Y Tsuchimochi</name></author><author><name sortKey="Ninomiya, T" uniqKey="Ninomiya T">T Ninomiya</name></author><author><name sortKey="Koga, T" uniqKey="Koga T">T Koga</name></author><author><name sortKey="Kitajima, A" uniqKey="Kitajima A">A Kitajima</name></author><author><name sortKey="Yamasaki, A" uniqKey="Yamasaki A">A Yamasaki</name></author></analytic></biblStruct><biblStruct><analytic><author><name sortKey="Penjor, T" uniqKey="Penjor T">T Penjor</name></author><author><name sortKey="Anai, T" uniqKey="Anai T">T Anai</name></author><author><name sortKey="Nagano, T" uniqKey="Nagano T">T Nagano</name></author><author><name sortKey="Matsumoto, R" uniqKey="Matsumoto R">R Matsumoto</name></author><author><name sortKey="Yamamoto, M" uniqKey="Yamamoto M">M Yamamoto</name></author></analytic></biblStruct><biblStruct><analytic><author><name sortKey="Li, X" uniqKey="Li X">X Li</name></author><author><name sortKey="Xie, R" uniqKey="Xie R">R Xie</name></author><author><name sortKey="Lu, Z" uniqKey="Lu Z">Z Lu</name></author><author><name sortKey="Zhou, Z" uniqKey="Zhou Z">Z Zhou</name></author></analytic></biblStruct><biblStruct><analytic><author><name sortKey="Deng, Z" uniqKey="Deng Z">Z Deng</name></author><author><name sortKey="La Malfa, S" uniqKey="La Malfa S">S La Malfa</name></author><author><name sortKey="Xie, Y" uniqKey="Xie Y">Y Xie</name></author><author><name sortKey="Xiong, X" uniqKey="Xiong X">X Xiong</name></author><author><name sortKey="Gentile, A" uniqKey="Gentile A">A Gentile</name></author></analytic></biblStruct><biblStruct><analytic><author><name sortKey="Garcia Lor, A" uniqKey="Garcia Lor A">A Garcia-Lor</name></author><author><name sortKey="Luro, F" uniqKey="Luro F">F Luro</name></author><author><name sortKey="Ollitrault, P" uniqKey="Ollitrault P">P Ollitrault</name></author><author><name sortKey="Navarro, L" uniqKey="Navarro L">L Navarro</name></author></analytic></biblStruct><biblStruct><analytic><author><name sortKey="Hang, L" uniqKey="Hang L">L Hang</name></author><author><name sortKey="Xiaoming, Y" uniqKey="Xiaoming Y">Y Xiaoming</name></author><author><name sortKey="Lianshu, Z" uniqKey="Lianshu Z">Z Lianshu</name></author><author><name sortKey="Hualin, Y" uniqKey="Hualin Y">Y Hualin</name></author><author><name sortKey="Lijun, C" uniqKey="Lijun C">C Lijun</name></author><author><name sortKey="Xiuxin, D" uniqKey="Xiuxin D">D Xiuxin</name></author></analytic></biblStruct><biblStruct><analytic><author><name sortKey="Curk, F" uniqKey="Curk F">F Curk</name></author><author><name sortKey="Ollitrault, F" uniqKey="Ollitrault F">F Ollitrault</name></author><author><name sortKey="Garcia Lor, A" uniqKey="Garcia Lor A">A Garcia-Lor</name></author><author><name sortKey="Luro, F" uniqKey="Luro F">F Luro</name></author><author><name sortKey="Navarro, L" uniqKey="Navarro L">L Navarro</name></author><author><name sortKey="Ollitrault, P" uniqKey="Ollitrault P">P Ollitrault</name></author></analytic></biblStruct><biblStruct><analytic><author><name sortKey="Lotfy, S" uniqKey="Lotfy S">S Lotfy</name></author><author><name sortKey="Luro, F" uniqKey="Luro F">F Luro</name></author><author><name sortKey="Carreel, F" uniqKey="Carreel F">F Carreel</name></author><author><name sortKey="Froelicher, Y" uniqKey="Froelicher Y">Y Froelicher</name></author><author><name sortKey="Rist, D" uniqKey="Rist D">D Rist</name></author><author><name sortKey="Ollitrault, P" uniqKey="Ollitrault P">P Ollitrault</name></author></analytic></biblStruct><biblStruct><analytic><author><name sortKey="Su, Hj" uniqKey="Su H">HJ Su</name></author><author><name sortKey="Ahogenhout, Sa" uniqKey="Ahogenhout S">SA AHogenhout</name></author><author><name sortKey="Al Sadi, Am" uniqKey="Al Sadi A">AM Al-Sadi</name></author><author><name sortKey="Kuo, Ch" uniqKey="Kuo C">CH Kuo</name></author></analytic></biblStruct><biblStruct><analytic><author><name sortKey="Hall, Ta" uniqKey="Hall T">TA Hall</name></author></analytic></biblStruct><biblStruct></biblStruct><biblStruct><analytic><author><name sortKey="Nei, M" uniqKey="Nei M">M Nei</name></author></analytic></biblStruct><biblStruct><analytic><author><name sortKey="Tamura, K" uniqKey="Tamura K">K Tamura</name></author><author><name sortKey="Stecher, G" uniqKey="Stecher G">G Stecher</name></author><author><name sortKey="Peterson, D" uniqKey="Peterson D">D Peterson</name></author><author><name sortKey="Filipski, A" uniqKey="Filipski A">A Filipski</name></author><author><name sortKey="Kumar, S" uniqKey="Kumar S">S Kumar</name></author></analytic></biblStruct><biblStruct><analytic><author><name sortKey="Abkenar, Aa" uniqKey="Abkenar A">AA Abkenar</name></author><author><name sortKey="Mardi, M" uniqKey="Mardi M">M Mardi</name></author><author><name sortKey="Matsumoto, R" uniqKey="Matsumoto R">R Matsumoto</name></author></analytic></biblStruct><biblStruct><analytic><author><name sortKey="Abkenar, Aa" uniqKey="Abkenar A">AA Abkenar</name></author><author><name sortKey="Isshiki, S" uniqKey="Isshiki S">S Isshiki</name></author><author><name sortKey="Tashino, Y" uniqKey="Tashino Y">Y Tashino</name></author></analytic></biblStruct><biblStruct><analytic><author><name sortKey="Roose, Ml" uniqKey="Roose M">ML Roose</name></author></analytic></biblStruct><biblStruct><analytic><author><name sortKey="Scora, Rw" uniqKey="Scora R">RW Scora</name></author></analytic></biblStruct><biblStruct><analytic><author><name sortKey="Herrero, R" uniqKey="Herrero R">R Herrero</name></author><author><name sortKey="Asins, Mj" uniqKey="Asins M">MJ Asìns</name></author><author><name sortKey="Carbonell, Ea" uniqKey="Carbonell E">EA Carbonell</name></author><author><name sortKey="Navarro, L" uniqKey="Navarro L">L Navarro</name></author></analytic></biblStruct><biblStruct><analytic><author><name sortKey="Ogawa, K" uniqKey="Ogawa K">K Ogawa</name></author><author><name sortKey="Kawasakib, A" uniqKey="Kawasakib A">A Kawasakib</name></author><author><name sortKey="Omurab, M" uniqKey="Omurab M">M Omurab</name></author><author><name sortKey="Yoshidab, T" uniqKey="Yoshidab T">T Yoshidab</name></author><author><name sortKey="Ikomab, Y" uniqKey="Ikomab Y">Y Ikomab</name></author><author><name sortKey="Yanob, M" uniqKey="Yanob M">M Yanob</name></author></analytic></biblStruct><biblStruct><analytic><author><name sortKey="Gulsen, O" uniqKey="Gulsen O">O Gulsen</name></author><author><name sortKey="Roose, Ml" uniqKey="Roose M">ML Roose</name></author></analytic></biblStruct><biblStruct><analytic><author><name sortKey="Ramadugu, C" uniqKey="Ramadugu C">C Ramadugu</name></author><author><name sortKey="Pfeil, Be" uniqKey="Pfeil B">BE Pfeil</name></author><author><name sortKey="Keremane, Ml" uniqKey="Keremane M">ML Keremane</name></author><author><name sortKey="Lee, Rf" uniqKey="Lee R">RF Lee</name></author><author><name sortKey="Maureira Butler, Ij" uniqKey="Maureira Butler I">IJ Maureira-Butler</name></author><author><name sortKey="Roose, Ml" uniqKey="Roose M">ML Roose</name></author></analytic></biblStruct><biblStruct><analytic><author><name sortKey="Pfeil, Be" uniqKey="Pfeil B">BE Pfeil</name></author><author><name sortKey="Crisp, Md" uniqKey="Crisp M">MD Crisp</name></author></analytic></biblStruct><biblStruct><analytic><author><name sortKey="Jung, Yh" uniqKey="Jung Y">YH Jung</name></author><author><name sortKey="Kwon, Hm" uniqKey="Kwon H">HM Kwon</name></author><author><name sortKey="Kang, Sh" uniqKey="Kang S">SH Kang</name></author><author><name sortKey="Kang, Jh" uniqKey="Kang J">JH Kang</name></author><author><name sortKey="Kim, Sc" uniqKey="Kim S">SC Kim</name></author></analytic></biblStruct><biblStruct><analytic><author><name sortKey="Cheng, Y" uniqKey="Cheng Y">Y Cheng</name></author><author><name sortKey="De Vicente, Mc" uniqKey="De Vicente M">MC De Vicente</name></author><author><name sortKey="Meng, H" uniqKey="Meng H">H Meng</name></author><author><name sortKey="Guo, W" uniqKey="Guo W">W Guo</name></author><author><name sortKey="Tao, N" uniqKey="Tao N">N Tao</name></author><author><name sortKey="Deng, X" uniqKey="Deng X">X Deng</name></author></analytic></biblStruct><biblStruct><analytic><author><name sortKey="Olivares Fuster, O" uniqKey="Olivares Fuster O">O Olivares-Fuster</name></author><author><name sortKey="Hernandez Garrido, M" uniqKey="Hernandez Garrido M">M Hernandez-Garrido</name></author><author><name sortKey="Guerri, J" uniqKey="Guerri J">J Guerri</name></author><author><name sortKey="Navarro, L" uniqKey="Navarro L">L Navarro</name></author></analytic></biblStruct><biblStruct><analytic><author><name sortKey="Garg, K" uniqKey="Garg K">K Garg</name></author><author><name sortKey="Green, P" uniqKey="Green P">P Green</name></author><author><name sortKey="Nickerson, Da" uniqKey="Nickerson D">DA Nickerson</name></author></analytic></biblStruct><biblStruct><analytic><author><name sortKey="Batley, J" uniqKey="Batley J">J Batley</name></author><author><name sortKey="Barker, G" uniqKey="Barker G">G Barker</name></author><author><name sortKey="O Ullivan, H" uniqKey="O Ullivan H">H O’Sullivan</name></author><author><name sortKey="Edwards, Kj" uniqKey="Edwards K">KJ Edwards</name></author><author><name sortKey="Edwards, D" uniqKey="Edwards D">D Edwards</name></author></analytic></biblStruct><biblStruct><analytic><author><name sortKey="Schroeder, H" uniqKey="Schroeder H">H Schroeder</name></author><author><name sortKey="Fladung, M" uniqKey="Fladung M">M Fladung</name></author></analytic></biblStruct><biblStruct><analytic><author><name sortKey="Lee, Yg" uniqKey="Lee Y">YG Lee</name></author><author><name sortKey="Jeong, N" uniqKey="Jeong N">N Jeong</name></author><author><name sortKey="Kim, Jh" uniqKey="Kim J">JH Kim</name></author><author><name sortKey="Lee, K" uniqKey="Lee K">K Lee</name></author><author><name sortKey="Kim, Kh" uniqKey="Kim K">KH Kim</name></author><author><name sortKey="Pirani, A" uniqKey="Pirani A">A Pirani</name></author></analytic></biblStruct><biblStruct><analytic><author><name sortKey="Vos, P" uniqKey="Vos P">P Vos</name></author><author><name sortKey="Uitdewilligen, J" uniqKey="Uitdewilligen J">J Uitdewilligen</name></author><author><name sortKey="Voorrips, R" uniqKey="Voorrips R">R Voorrips</name></author><author><name sortKey="Visser, R" uniqKey="Visser R">R Visser</name></author><author><name sortKey="Van Eck, H" uniqKey="Van Eck H">H Van Eck</name></author></analytic></biblStruct><biblStruct><analytic><author><name sortKey="Wu, Ga" uniqKey="Wu G">GA Wu</name></author><author><name sortKey="Prochnik, S" uniqKey="Prochnik S">S Prochnik</name></author><author><name sortKey="Jenkins, J" uniqKey="Jenkins J">J Jenkins</name></author><author><name sortKey="Salse, J" uniqKey="Salse J">J Salse</name></author><author><name sortKey="Hellsten, U" uniqKey="Hellsten U">U Hellsten</name></author><author><name sortKey="Murat, F" uniqKey="Murat F">F Murat</name></author></analytic></biblStruct></listBibl></div1></back></TEI><affiliations><list><country><li>Espagne</li><li>France</li><li>Tunisie</li></country><region><li>Guadeloupe</li><li>Province de Valence</li></region><settlement><li>Guadeloupe France</li></settlement></list><tree><country name="Tunisie"><noRegion><name sortKey="Oueslati, Amel" sort="Oueslati, Amel" uniqKey="Oueslati A" first="Amel" last="Oueslati">Amel Oueslati</name></noRegion><name sortKey="Baraket, Ghada" sort="Baraket, Ghada" uniqKey="Baraket G" first="Ghada" last="Baraket">Ghada Baraket</name><name sortKey="Salhi Hannachi, Amel" sort="Salhi Hannachi, Amel" uniqKey="Salhi Hannachi A" first="Amel" last="Salhi-Hannachi">Amel Salhi-Hannachi</name></country><country name="France"><region name="Guadeloupe"><name sortKey="Oueslati, Amel" sort="Oueslati, Amel" uniqKey="Oueslati A" first="Amel" last="Oueslati">Amel Oueslati</name></region><name sortKey="Ollitrault, Patrick" sort="Ollitrault, Patrick" uniqKey="Ollitrault P" first="Patrick" last="Ollitrault">Patrick Ollitrault</name></country><country name="Espagne"><region name="Province de Valence"><name sortKey="Ollitrault, Frederique" sort="Ollitrault, Frederique" uniqKey="Ollitrault F" first="Frederique" last="Ollitrault">Frederique Ollitrault</name></region><name sortKey="Navarro, Luis" sort="Navarro, Luis" uniqKey="Navarro L" first="Luis" last="Navarro">Luis Navarro</name></country></tree></affiliations></record>Pour manipuler ce document sous Unix (Dilib)
EXPLOR_STEP=$WICRI_ROOT/Wicri/Bois/explor/OrangerV1/Data/Main/Exploration
HfdSelect -h $EXPLOR_STEP/biblio.hfd -nk 000022 | SxmlIndent | more
Ou
HfdSelect -h $EXPLOR_AREA/Data/Main/Exploration/biblio.hfd -nk 000022 | SxmlIndent | more
Pour mettre un lien sur cette page dans le réseau Wicri
{{Explor lien
|wiki= Wicri/Bois
|area= OrangerV1
|flux= Main
|étape= Exploration
|type= RBID
|clé= PMC:4991024
|texte= Towards a molecular taxonomic key of the Aurantioideae subfamily using chloroplastic SNP diagnostic markers of the main clades genotyped by competitive allele-specific PCR
}}
Pour générer des pages wiki
HfdIndexSelect -h $EXPLOR_AREA/Data/Main/Exploration/RBID.i -Sk "pubmed:27539067" \
| HfdSelect -Kh $EXPLOR_AREA/Data/Main/Exploration/biblio.hfd \
| NlmPubMed2Wicri -a OrangerV1
| This area was generated with Dilib version V0.6.25. |  |